What event(s) occur during anaphase? The spindle pulls on the duplicated chromosomes. The duplicated chromosomes split. The DNA forms into chromosomes. The nucleus dissolves.

(A) (i) Describe TWO disadvantages of using Replicate Organism Detection And Counting (RODAC) plate during a surface swab. (ii) Explain TWO purposes of conducting a hand swab test. 12 m

8 Which of the following is a type of universal media? (A) MacConkey Agar. (B) Baird-Parker Agar. (C) \( \quad \) Mannitol Salt Agar. (D) Trypticase Soy Agar. 9

(C) State TWO ways in which fermentation process helps to improve [2 marks] quality of food products.

Glycerol a) Name the: (i) Process \( X \) (ii) Enzyme \( Y \) (iii) Molecule Z

(A) State ONE example of selective media that can be used to isolate [3 marks] EACH of the following microorganisms. (i) Gram positive bacteria. (ii) Staphylococci spp. (iii) Gram negative bacteria.