Ane of the best ways to break the chain of infection is to A) get boosters for immunizations 6 months early. B) avoid disinfectant agents when cleaning your house. C) drink only fluoridated water. D) wash your hands frequently.

Calculate the cfu/cm \( \mathrm{cm}^{2} \) for a tested surface of 4 cm in length and 3 cm in width if the number of colonies on the agar plate is 96 cfu. (A) \( 8 \mathrm{cfu} / \mathrm{cm}^{2} \) (B) \( 12 \mathrm{cmu} / \mathrm{cm}^{2} \). (C) \( 72 \mathrm{cmu} / \mathrm{cm}^{2} \) (D) \( 128 \mathrm{cmu} / \mathrm{cm}^{2} \)

(B) State TWO ways in which flagellum helps a microorganism survive in an open environment. (2 ma All rights reserved. No part of this publication may be reproduced, stored in a retrieval sys transmitted in any form or by any means, electronic, mechanical, photocopying, reco otherwise, without the prior written permission of the Institute of Technical Education, Singapo Institute of Technical Education has made every effort to trace copyright holders, but if inadvertently overlooked any, we will be pleased to make the necessary arrangements a

(B) (i) Explain THREE precaution steps during decontamination of biohazard wastes in bag using an autoclave. (ii) Describe TWO materials that will remain undamaged after autoclaving.

34 (A) (i) Explain ONE purpose of air monitoring. (ii) Describe TWO factors that will affect air quality.

23 Which one of the following microorganisms moves using flagellum? (A) Amoeba. (B) Euglena. (C) Paramecium. (D) Plasmodium. Which one of the following is NOT a heterotrophic microorganism? \( \begin{array}{ll}\text { (A) } \quad \text { Diatoms. } \\ \text { (B) } \quad \text { Sarcodines. } \\ \text { (C) } \quad \text { Sporozoans. } \\ \text { (D) }\end{array} \)