Question 7 (2 points) \( \begin{array}{l}\text { Aisten } \\ \text { All of the following are complex carbohydrates EXCEPT } \\ \text { white bread } \\ \text { fruit juice } \\ \text { whole wheat bread } \\ \text { cereal } \\ \text { Question } 8 \text { (2 points) }\end{array} \)

34 (A) (i) Explain ONE purpose of air monitoring. (ii) Describe TWO factors that will affect air quality.

(B) (i) Explain THREE precaution steps during decontamination of biohazard wastes in bag using an autoclave. (ii) Describe TWO materials that will remain undamaged after autoclaving.

8 Which of the following is a type of universal media? (A) MacConkey Agar. (B) Baird-Parker Agar. (C) \( \quad \) Mannitol Salt Agar. (D) Trypticase Soy Agar. 9

Calculate the cfu/cm \( \mathrm{cm}^{2} \) for a tested surface of 4 cm in length and 3 cm in width if the number of colonies on the agar plate is 96 cfu. (A) \( 8 \mathrm{cfu} / \mathrm{cm}^{2} \) (B) \( 12 \mathrm{cmu} / \mathrm{cm}^{2} \). (C) \( 72 \mathrm{cmu} / \mathrm{cm}^{2} \) (D) \( 128 \mathrm{cmu} / \mathrm{cm}^{2} \)

(A) State ONE example of selective media that can be used to isolate [3 marks] EACH of the following microorganisms. (i) Gram positive bacteria. (ii) Staphylococci spp. (iii) Gram negative bacteria.