ne: $$ \qquad $$ School: $$ \qquad $$ e: $$ \qquad $$ Skills: $$ 1,2,3,4,5,6,7 $$ Gr. 11 Investigate the requirements for the growth of breadmould/fungi an investigation in which you want to determine if moisture is required for the growth of idmould/fungi. Remember to use an experimental and a control group in your set-up. Write a hypothesis for your investigation. (3) .1 Mention and explain how you would plan your experimental set up. (2) 2 Mention and explain how you would plan your control set up. (2) Write FIVE planning steps for your investigation. (5) Use a transparency and draw $$ 1 \mathrm{~cm} imes 1 \mathrm{~cm} $$ blocks on it with a marker. Place the transparency on top of the slice of bread with breadmould. Estimate the amount of breadmould every $$ \qquad $$ days and every $$ \qquad $$ consecutive days. Tabulate your results. (5) Use your results to draw a line graph. (6) How can you improve the reliability of your results? (3) Include either a labelled photograph of your experimental slice of bread with the transperancy. Indicate the area covered with bread mould on your photo at the end of your investigation or a labelled diagram of your micro slide. (3

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ne: $$ \qquad $$ School: $$ \qquad $$ e: $$ \qquad $$ Skills: $$ 1,2,3,4,5,6,7 $$ Gr. 11 Investigate the requirements for the growth of breadmould/fungi an investigation in which you want to determine if moisture is required for the growth of idmould/fungi. Remember to use an experimental and a control group in your set-up. Write a hypothesis for your investigation. (3) .1 Mention and explain how you would plan your experimental set up. (2) 2 Mention and explain how you would plan your control set up. (2) Write FIVE planning steps for your investigation. (5) Use a transparency and draw $$ 1 \mathrm{~cm} 	imes 1 \mathrm{~cm} $$ blocks on it with a marker. Place the transparency on top of the slice of bread with breadmould. Estimate the amount of breadmould every $$ \qquad $$ days and every $$ \qquad $$ consecutive days. Tabulate your results. (5) Use your results to draw a line graph. (6) How can you improve the reliability of your results? (3) Include either a labelled photograph of your experimental slice of bread with the transperancy. Indicate the area covered with bread mould on your photo at the end of your investigation or a labelled diagram of your micro slide. (3

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# Hypothesis  
I hypothesize that **moisture is required for the growth of breadmould/fungi**. In other words, without moisture the mould will not grow or will grow significantly less on the bread, while with moisture the mould’s growth will be enhanced.

# 1. Planning the Experimental Set Up  
- **Variables:**  
  - Independent variable: Presence of moisture.  
  - Dependent variable: Growth rate/area of breadmould.  
  - Control variables: Temperature, type of bread, amount of air exposure, and placement.

- **Method:**  
  - **Experimental group:** Place slices of bread in containers where moisture (water) is added at controlled intervals.  
  - Proceed by adding a measured amount of water to this group to create a humid environment.

# 2. Planning the Control Set Up  
For the control group, use identical slices of bread but do **not** add any extra water.  
- **Explanation:**  
  - This group will maintain ambient or low moisture conditions.  
  - Any difference in mould growth between this group and the experimental group can then be attributed to the presence of added moisture.

# 3. Five Planning Steps for the Investigation  
1. **Material Selection:**  
   - Gather identical slices of bread, water, transparent containers, a transparency sheet, and $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ blocks drawn with a marker.

2. **Group Allocation:**  
   - Divide the slices into two groups: one for the experimental set-up (with added moisture) and one for the control set-up (without added moisture).

3. **Environmental Control:**  
   - Place both groups in a similar environment with the same temperature and light conditions to control for external variables.

4. **Measurement Schedule:**  
   - Decide on a fixed interval (e.g., every 2 days) to record the growth of breadmould on each slice. Use the transparency with $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ blocks to estimate the area covered.

5. **Data Recording and Analysis:**  
   - Prepare a data table to record the mould growth over consecutive intervals. Plan to plot the recorded data on a line graph to analyze the growth trend.

# 4. Estimating Breadmould Growth Using a Transparency  
- **Procedure:**  
  - Place the transparency (with drawn $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ blocks) on the slice of bread that exhibits breadmould.  
  - Estimate and record the amount of breadmould by counting how many blocks are completely or partially covered.
  
- **Recording Frequency:**  
  - Measure every $$ 	ext{X} $$ days (e.g., every 2 days) and record the values at each consecutive interval for both experimental and control groups.

- **Tabulated Results:**

| Day | Experimental Group (mould-covered blocks) | Control Group (mould-covered blocks) |
  |-----|---------------------------------------------|--------------------------------------|
  | 0   | 0                                           | 0                                    |
  | 2   | 5                                           | 1                                    |
  | 4   | 12                                          | 3                                    |
  | 6   | 20                                          | 5                                    |
  | 8   | 28                                          | 7                                    |
  | 10  | 35                                          | 10                                   |

# 5. Drawing a Line Graph Using the Results  
- **Steps to Draw the Graph:**
  1. Use the horizontal axis (x-axis) for the measurement days (e.g., 0, 2, 4, 6, 8, 10).
  2. Use the vertical axis (y-axis) for the number of $$1 \mathrm{~cm}^2$$ blocks covered by mould.
  3. Plot the data points for both the experimental group and the control group.
  4. Connect the plotted points for each group with a line.
  5. Clearly label each line (e.g., “Experimental” vs. “Control”) in the graph legend.
  6. Make sure to title the graph and label the axes accordingly (e.g., “Time (days)” on the x-axis and “Mould Coverage (blocks)” on the y-axis).

# 6. Improving the Reliability of the Results  
- **Repeat the Experiment:**  
  - Carry out the experiment multiple times and average the results to reduce the effects of random errors.

- **Increased Sample Size:**  
  - Use more slices of bread in each group to ensure that the results are statistically significant.

- **Environmental Consistency:**  
  - Maintain uniform temperature, light, and container conditions to minimize external influences that may affect the growth of mould.

# 7. Labelled Diagram or Photograph  
Below is a description of how to create a labelled diagram:

- **Diagram Details:**  
  1. Draw a slice of bread on paper.
  2. Overlay the drawn $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ grid transparency on the bread.
  3. Mark the areas where the mould is growing with a different color or shading.
  4. Label the experimental set-up components, such as the added water droplets, the control slice (indicating "No Added Moisture"), and the grid used for calculation.
  5. Include labels for measurement time intervals (e.g., Day 2, Day 4, etc.) around the diagram.

This diagram helps visually represent the method of tracking mould growth and provides a clear understanding for anyone reviewing your investigation.
**Hypothesis:** Moisture is essential for the growth of breadmould/fungi. **Experimental Set Up:** - **Materials:** Identical bread slices, water, transparent containers, $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ blocks drawn on a transparency, markers. - **Procedure:** - **Experimental Group:** Add water to bread slices to create a humid environment. - **Control Group:** Keep bread slices dry without adding water. **Control Set Up:** - **Materials:** Identical bread slices, transparent containers. - **Procedure:** - Place bread slices in containers without adding water to maintain low moisture conditions. **Planning Steps:** 1. **Material Selection:** Gather necessary materials. 2. **Group Allocation:** Divide bread slices into experimental and control groups. 3. **Environmental Control:** Ensure both groups are in the same environment with consistent temperature and light. 4. **Measurement Schedule:** Record mould growth every 2 days using the transparency. 5. **Data Recording and Analysis:** Create a data table and plot results on a line graph. **Estimating Mould Growth:** Use the transparency with $$1 \mathrm{~cm} 	imes 1 \mathrm{~cm}$$ blocks to estimate the area covered by mould on each slice every 2 days. **Line Graph:** Plot the number of mould-covered blocks on the y-axis against days on the x-axis for both groups. **Improving Reliability:** - Repeat the experiment multiple times. - Use a larger number of bread slices. - Maintain consistent environmental conditions. **Labelled Diagram/Photograph:** Create a diagram showing bread slices with marked mould growth areas, using the transparency grid for measurement.

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