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Experiment II: For the Gel Photo at the end of the complete Gel Electrophoresis animation: 1. How many bands were in lane 3 (EcoRI)? [Select ] 2. How many bands were in lane 5 (BamHI)? [ Select ] 3. How many bands were in lane 7 (EcorRI)? [ Select ]

Ask by Riley Mann. in the United States
Feb 25,2025

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Answer

To determine the number of bands in each lane, carefully examine the Gel Photo for lanes 3, 5, and 7. Count the distinct bands in each lane and select the corresponding number for each question.

Solution

To provide a detailed solution, I need to clarify that I do not have access to visual content such as the Gel Photo from the Gel Electrophoresis animation. However, I can guide you on how to analyze a gel electrophoresis image to determine the number of bands in each lane. 1. **Identify the Lanes**: Look at the gel image and locate lane 3, lane 5, and lane 7. Each lane corresponds to a different restriction enzyme (EcoRI or BamHI). 2. **Count the Bands**: For each lane: - **Lane 3 (EcoRI)**: Count the distinct bands present in this lane. - **Lane 5 (BamHI)**: Count the distinct bands present in this lane. - **Lane 7 (EcoRI)**: Count the distinct bands present in this lane. 3. **Select the Number of Bands**: Based on your observations, select the number of bands for each lane. If you can provide the specific counts or any additional details from the gel image, I can help you interpret the results further.

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Bonus Knowledge

Electrophoresis is like a reality show for DNA fragments, where they race through a gel matrix, and their size determines how quickly they reach the finish line. Lane 3, containing EcoRI digested DNA, should show multiple bands depending on the sample complexity; often, you might expect to see 4-5 distinctive bands. For lane 5 with BamHI, this enzyme typically generates fewer bands since it recognizes a different sequence, usually around 2-3 bands. Lane 7 with EcoRI can again showcase multiple bands if it's a mix of DNA fragments since EcoRI is a common enzyme for gene cloning and known for cutting at specific sequences in a sample! If you want to dive deeper into the science behind this, check out the fascinating world of restriction enzymes and their applications in genetic engineering. Books like "Molecular Cloning: A Laboratory Manual" by Sambrook and Russell provide great insights and experimental techniques that are essential for any aspiring molecular biologist!

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