Experiment II: For the Gel Photo at the end of the complete Gel Electrophoresis animation: 1. How many bands were in lane 3 (EcoRI)? [Select ] 2. How many bands were in lane 5 (BamHI)? [ Select ] 3. How many bands were in lane 7 (EcorRI)? [ Select ]
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Bonus Knowledge
Electrophoresis is like a reality show for DNA fragments, where they race through a gel matrix, and their size determines how quickly they reach the finish line. Lane 3, containing EcoRI digested DNA, should show multiple bands depending on the sample complexity; often, you might expect to see 4-5 distinctive bands. For lane 5 with BamHI, this enzyme typically generates fewer bands since it recognizes a different sequence, usually around 2-3 bands. Lane 7 with EcoRI can again showcase multiple bands if it's a mix of DNA fragments since EcoRI is a common enzyme for gene cloning and known for cutting at specific sequences in a sample! If you want to dive deeper into the science behind this, check out the fascinating world of restriction enzymes and their applications in genetic engineering. Books like "Molecular Cloning: A Laboratory Manual" by Sambrook and Russell provide great insights and experimental techniques that are essential for any aspiring molecular biologist!