Question
Experiment II: Which of the following restriction enzymes create sticky ends? (Select all th apply.) EcoRI Smal BamHI
Ask by Donnelly Washington. in the United States
Feb 25,2025
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Answer
EcoRI and BamHI create sticky ends.
Solution
EcoRI and BamHI create sticky ends. Smal (SmaI) creates blunt ends.
EcoRI cuts at GAATTC producing a 5' overhang and BamHI cuts at GGATCC also producing a 5' overhang. SmaI, on the other hand, produces blunt ends when it cuts at CCCGGG.
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EcoRI and BamHI are both restriction enzymes that create sticky ends, which means they cut the DNA in a staggered manner, leaving overhanging ends that can easily pair with complementary sequences. This makes them super helpful for cloning and recombinant DNA technology because those sticky ends can facilitate the ligation of DNA fragments. On the other hand, Smal creates blunt ends, which means it cuts straight across the DNA helix, resulting in fragments that are harder to join together compared to the sticky ends. Blunt ends can still be ligated, but often require more precise conditions and can lead to lower efficiency in cloning experiments.
