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Experiment I: For each of the following, please choose the appropriate function of the substance. Liquid Detergent in the Extraction Buffer [Choose] [ Choose ] Salt in the Extraction Buffer Alcohol in the Extraction Buffer \( \begin{array}{l}\text { [ Choose ] } \\ \text { Negates the negative charges in the DNA } \\ \text { Enzymes that cut DNA } \\ \text { Source of DNA } \\ \text { Precipitates the DNA } \\ \text { Dissolves the plasma membrane and binds proteins and carbs }\end{array} \) [ Choose ] DNases

Ask by Reed Washington. in the United States
Feb 25,2025

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Answer

- **Liquid Detergent**: Dissolves the plasma membrane and binds proteins and carbohydrates. - **Salt**: Negates the negative charges in the DNA. - **Alcohol**: Precipitates the DNA. - **DNases**: Enzymes that cut DNA.

Solution

To determine the appropriate function of each substance in the extraction buffer, let's analyze each component: 1. **Liquid Detergent in the Extraction Buffer**: - The function of liquid detergent is to **dissolve the plasma membrane and bind proteins and carbohydrates**. Detergents disrupt lipid bilayers, allowing the extraction of cellular components. 2. **Salt in the Extraction Buffer**: - The function of salt is to **negate the negative charges in the DNA**. Salt helps to stabilize the DNA by neutralizing the charges on the phosphate backbone, which facilitates the precipitation of DNA. 3. **Alcohol in the Extraction Buffer**: - The function of alcohol is to **precipitate the DNA**. Alcohol (usually ethanol or isopropanol) is used to precipitate DNA from the solution, allowing it to be collected. 4. **DNases**: - The function of DNases is to **enzymes that cut DNA**. DNases are enzymes that degrade DNA, which is important to prevent contamination during the extraction process. Now, let's summarize the choices: - **Liquid Detergent**: Dissolves the plasma membrane and binds proteins and carbohydrates. - **Salt**: Negates the negative charges in the DNA. - **Alcohol**: Precipitates the DNA. - **DNases**: Enzymes that cut DNA. If you need further assistance or clarification, feel free to ask!

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Bonus Knowledge

Liquid detergent acts as a surfactant that dissolves the plasma membrane of cells, effectively breaking down lipid bilayers and releasing the cellular contents, including DNA. It's like giving the cells a gentle nudge to let their goodies out! Salt, on the other hand, negates the negative charges on the DNA molecules, which helps in the process of DNA precipitation by ensuring that the DNA clumps together, making it easier to isolate and work with. Think of it as getting the DNA cozy enough to form a snuggly little ball!

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