What is a polypeptide a sequence of? What kind of bond holds this sequence together? What two things does a tRNA transfer or carry to the ribosome? How do you know where to begin translating? Put a box around what tells you where to begin in \#1 and \#2. How do you know when to stop translating? Put a star by what tells you where to end in \#1 and 2 . In what two places in the cell can translation occur? W. W.

A polypeptide is a sequence of amino acids linked together by peptide bonds. These peptide bonds are formed through a dehydration reaction between the carboxyl group of one amino acid and the amino group of another, resulting in a covalent connection that creates a long chain of amino acids—the building blocks of proteins! tRNA, or transfer RNA, carries two key components to the ribosome: a specific amino acid that corresponds to its anticodon and the information for which amino acid the codon specifies. The anticodon is a three-nucleotide sequence that pairs with the mRNA's codon, ensuring the correct amino acid is added to the growing polypeptide chain as the ribosome synthesizes proteins. When it comes to starting translation, the ribosome recognizes the **start codon (AUG)**, which codes for methionine, marking the beginning of the polypeptide synthesis. As for stopping, translation concludes upon encountering a **stop codon (UAA, UAG, or UGA)**, which signals that the completed polypeptide chain is ready to be released. Translation occurs primarily in two areas of the cell: in the **cytoplasm**, where free ribosomes can translate proteins destined for various cellular functions, and on the **rough endoplasmic reticulum (RER)**, where ribosomes are bound and translate proteins that are typically secreted or embedded in membranes.