INSTRUCTIONS AND INFORMATION. 1. Materials for the practical task per group. - Benedict's Reagent \( { }^{1} \) - 4Test tubes per group - Test tube rack - Water bath or hotplate - Beaker - Thermometer - Drink sample (Mineral water, flavoured mineral water, fresh orange juice/ grape juice, Coke). - 5 ml droppers / plastic pipettes (NB droppers must allow you to measure the volume of sample) - Timer - Tongs 2. The task will test the following skill to address Specific Aim 2 of Life Sciences: - Following instruction - Handling equipment/ Apparatus - Making Observation - Recording Data - Interpretation of data - Confidential Information - This is for official consumption If your school doesn't have Benedict's reagent, follow the instruction below to prepare the solution. HOW TO PREPARE BENEDICT SOLUTION: Chemicals required: 2. 100 g Sodium carbonate \( \left(\mathrm{Na}_{2} \mathrm{CO}_{3}\right) \) - Any store. 3. 173 g Sodium Citrate \( \left(\mathrm{Na}_{3} \mathrm{C}_{6} \mathrm{H}_{5} \mathrm{O}_{7}\right) \)

To prepare Benedict's solution, follow these steps carefully: ### Materials Needed: 1. **Sodium Carbonate** \( \left(\mathrm{Na}_{2} \mathrm{CO}_{3}\right) \) - 100 g 2. **Sodium Citrate** \( \left(\mathrm{Na}_{3} \mathrm{C}_{6} \mathrm{H}_{5} \mathrm{O}_{7}\right) \) - 173 g 3. **Distilled Water** - Sufficient quantity to make up the solution 4. **Beaker** - For mixing 5. **Stirring Rod** - For mixing the solution 6. **Scale** - To measure the chemicals accurately ### Procedure: 1. **Measure the Chemicals:** - Weigh out 100 g of Sodium Carbonate \( \left(\mathrm{Na}_{2} \mathrm{CO}_{3}\right) \). - Weigh out 173 g of Sodium Citrate \( \left(\mathrm{Na}_{3} \mathrm{C}_{6} \mathrm{H}_{5} \mathrm{O}_{7}\right) \). 2. **Mix the Chemicals:** - In a beaker, add the 100 g of Sodium Carbonate. - Add the 173 g of Sodium Citrate to the beaker. 3. **Add Distilled Water:** - Gradually add distilled water to the beaker while stirring the mixture with a stirring rod. - Continue adding water until the total volume of the solution reaches 1 liter. 4. **Stir Thoroughly:** - Ensure that both Sodium Carbonate and Sodium Citrate are completely dissolved in the water. Stir the solution thoroughly to achieve a uniform mixture. 5. **Storage:** - Transfer the prepared Benedict's solution into a clean, labeled container for storage. - Store the solution in a cool, dark place to maintain its effectiveness. ### Important Notes: - Benedict's solution is used to test for reducing sugars. Ensure to follow safety protocols while handling chemicals. - If you are unsure about any step, consult with a teacher or supervisor before proceeding. This prepared solution can now be used for your practical task involving the testing of various drink samples.